Explore BrainMass
Share

Explore BrainMass

    mRNA analysis in the lab

    This content was COPIED from BrainMass.com - View the original, and get the already-completed solution here!

    Describe 3 ways of measuring changes in mRNA expression and provide an explanation of how each one works. Does the method measure mRNA synthesis, degradation, or levels?

    © BrainMass Inc. brainmass.com October 9, 2019, 3:28 pm ad1c9bdddf
    https://brainmass.com/biology/research-methods-and-experimental-design/mrna-analysis-lab-4886

    Solution Preview

    1. Nuclear run-on assay: Measures relative amounts of mRNA synthesis.
    a. Isolate nuclei (keep cold to inhibit transcription) at the different times being examined (e.g., lengths of treatment).
    b. Add 32P-labeled rNTPs, allow transcription of nascent RNAs to continue.
    c. Hybridize RNA to complementary DNA of interest.
    d. Measure level of radioactivity of the DNA-RNA hybrids
    ...

    Solution Summary

    An mRNA analysis in the lab are determined. The expert describes three ways of measuring change in mRNA expression. How each one works is provided.

    $2.19