Purchase Solution

mRNA analysis in the lab

Not what you're looking for?

Ask Custom Question

Describe 3 ways of measuring changes in mRNA expression and provide an explanation of how each one works. Does the method measure mRNA synthesis, degradation, or levels?

Purchase this Solution

Solution Summary

An mRNA analysis in the lab are determined. The expert describes three ways of measuring change in mRNA expression. How each one works is provided.

Solution Preview

1. Nuclear run-on assay: Measures relative amounts of mRNA synthesis.
a. Isolate nuclei (keep cold to inhibit transcription) at the different times being examined (e.g., lengths of treatment).
b. Add 32P-labeled rNTPs, allow transcription of nascent RNAs to continue.
c. Hybridize RNA to complementary DNA of interest.
d. Measure level of radioactivity of the DNA-RNA hybrids

Purchase this Solution

Free BrainMass Quizzes
Hemophilia: Fact or Fiction

Do you know the truth about hemophilia? Test your knowledge here.

Breast Milk and Breastfeeding

How much do you know about breast milk and breastfeeding? Double check your knowledge level with this quiz!

Infant Development: Sleep

How much do you know about infant sleep? Test your knowledge with this quiz.

The Transfer of Energy in an Ecosystem

This quiz will assess your knowledge of how energy is transferred in an ecosystem and the different levels of trophic organization.

BioChemistry Basics

This Quiz will test your knowledge of the amino acids used in biological systems