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    mRNA analysis in the lab

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    Describe 3 ways of measuring changes in mRNA expression and provide an explanation of how each one works. Does the method measure mRNA synthesis, degradation, or levels?

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    1. Nuclear run-on assay: Measures relative amounts of mRNA synthesis.
    a. Isolate nuclei (keep cold to inhibit transcription) at the different times being examined (e.g., lengths of treatment).
    b. Add 32P-labeled rNTPs, allow transcription of nascent RNAs to continue.
    c. Hybridize RNA to complementary DNA of interest.
    d. Measure level of radioactivity of the DNA-RNA hybrids

    Solution Summary

    An mRNA analysis in the lab are determined. The expert describes three ways of measuring change in mRNA expression. How each one works is provided.