The table below indicates approximate values for these at 25C. For reference, the delta H(ionization) for Tris is 47kJ/mole.
Group deta H (ionization)(kJ/mole)
C- term 0
How will the net charge of a typical protein change upon lowering the temperature from 37C to 4C, based on this information?
What can be done experimentally to compensate for this?© BrainMass Inc. brainmass.com May 20, 2020, 12:59 pm ad1c9bdddf
Temperature has a marked effect on ionization, the extent of which depends on the heat of ionization, deltaH, of the various charged groups. In order to understand this, we need a relationship between pKa and deltaH.
The relationship between the change in the pKa and the change in temperature is given by a derivative of the Gibbs-Helmholtz equation:
ln(K1/K2) = (-deltaH/R)(1/T2 - ...
This solution is provided in 285 words. It discusses ionization and how this is related to temperature. References are also included to enhance learning.