Explore BrainMass
Share

The net total charge of a protein

This content was STOLEN from BrainMass.com - View the original, and get the already-completed solution here!

What is the net total charge on the following protein at pH7 and at pH2? Two answers
Lys-Glu-Phe-Lys

© BrainMass Inc. brainmass.com October 24, 2018, 5:43 pm ad1c9bdddf
https://brainmass.com/chemistry/biological-chemistry/the-net-total-charge-of-a-protein-18866

Solution Summary

The solution is provided in an attachment with two diagrams.

$2.19
See Also This Related BrainMass Solution

Galactose represser protein

The galactose represser protein from E. coli has a pI of about 5.9. While purification protocols were being designed, it was found to bind to a Mono-S column at pH values of 7 and below. (Mono-S columns have S-type sulfonic acid groups attached to the resin and are strong cation exchangers.)

What is unusual about this observation, and explain these results?

View Full Posting Details