The net total charge of a protein
What is the net total charge on the following protein at pH7 and at pH2? Two answers
Lys-Glu-Phe-Lys
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The solution is provided in an attachment with two diagrams.
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Galactose represser protein
The galactose represser protein from E. coli has a pI of about 5.9. While purification protocols were being designed, it was found to bind to a Mono-S column at pH values of 7 and below. (Mono-S columns have S-type sulfonic acid groups attached to the resin and are strong cation exchangers.)
What is unusual about this observation, and explain these results?
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