What is the net total charge on the following protein at pH7 and at pH2? Two answers
The solution is provided in an attachment with two diagrams.
Galactose represser protein
The galactose represser protein from E. coli has a pI of about 5.9. While purification protocols were being designed, it was found to bind to a Mono-S column at pH values of 7 and below. (Mono-S columns have S-type sulfonic acid groups attached to the resin and are strong cation exchangers.)
What is unusual about this observation, and explain these results?View Full Posting Details