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The effects of pH and temperature

The effects of pH and temperature were studied for an enzyme-catalyzed reaction. The following results were obtained.

a. How do (1) temperature and (2) pH affect the activity of this enzyme? lii your answer, include a discussion of the relationship between the structure and the function of this enzyme, as well as a discussion of how structure and function of enzymes are affected by temperature and pH.

b. Describe a controlled experiment that could have produced the data shown for either temperature or pH. Be sure to state the hypothesis that was tested here.

Help is given in these areas.

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a. Look first at the temperature graph. You can see that there is high enzyme activity near the middle of the graph. The enzyme activity is lower at lower temperatures and also lower at higher temperatures. When you see a graph that has a shape like this, you can guess that the enzyme has a specific temperature that it is most effective at. That temperature will be the peak in the graph.

Now look at the pH graph. You see that it has a similar shape to the temperature graph. So you can assume that the enzyme has a pH that it works best at, and that pH is the center of the graph.

The temperature where the graphs peaks is called the optimum temperature, and the pH where that graph peaks is called the optimum pH.

Why is there an optimum temp and pH? Because enzymes are ...

Solution Summary

The effects of pH and temperature are noted.