antibiotics and plasmid replication

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  The E. coli ORI region acts as an origin of replication when the plasmid is in these cells, and it is the region where replication of the plasmid starts.

• DNA Transformation

  This period allows for replication of plasmid as well as the transcription and translation of the antibiotic resistance gene mRNA before challenging the cells with antibiotics. 8) 8) Obtain two ampicillin (AMP) and two nutrient agar (NA) plates.

• Vectors

  "A typical plasmid vector contains a polylinker (which can recognize several different restriction enzymes), an antibiotic resistance gene for selective amplification, and a replication origin (ORI) for proliferation in the host cell".

• Growth of competent cells on LB plate with ampicillin

  The other consisted of plating cells without plasmid on LB and ampicillin plate. What is expected the controls, will you see growth or no growth and why? We have competent cells without plasmid.

• DNA Mutation Sequences and Their Impact on DNA replication

  581325 DNA mutation sequences and how they impact DNA replication 1. What is the mutation, how does this mutation impact DNA replication? 2. How was the relationship between the mutation and the disease discovered? 3.

• Bacterial Genetics and Replication

  I believed that this conjugation involve a donor bacterium that contain a "conjugative plasmids" and a recipient bacterium that does not. (2) A " conjugative plasmid contains all the genes necessary for the plasmid to transmit itself to another bacterium

• Concept map of antimicrobials

  They either attach DNA replication and transcription, or cell wall synthesis or protein synthesis.

• Microbes - Antibiotics

  In 1968, 12,500 Guatemalans died in an epidemic of Shigella diarrhea, caused by a microbe harboring a plasmid that conferred resistances to four antibiotics!

• Antibiotic development and resistance

  replication.