Purchase Solution

Intracellularly recording from pyramidal cells in a mouse

Not what you're looking for?

Ask Custom Question

I am recording intracellularly from the neocortex of a mouse. If I place my electrode on one set of pyramidal neurons (Layer 2) and add fenfluramine (a serotonin uptake blocker), whithin 5 minutes the neurons produce GREATER amount of action potentials. But when I place fenfluramine while recording from another set of pyramidal cells (Layer 5 for example), the neurons showed a DECREASE in AP's (action potentials) after 5 minutes of treatment. How is this possible for one drug to affect similar cells in completely different methods?

Please offer any feedback - if you feel that I haven't explained the question well enough, or if you aren't certain what I am looking for, please let me know. I am looking for help getting started on this and am hoping that you can provide some guidance.

Purchase this Solution

Solution Summary

The intracellular recording from pyramidal cells in a mouse is determined.

Solution Preview

Hi There,

Sorry for the delay in responding, but I have been experiencing some Microsoft Runtime Errors with internet explorer.

I found your posting to be very interesting. My approach to neuronal physiology is from how changes in neuronal physiology ultimately affect (or effect) the animal's behavior. Namely, my approach to this posting was in considering how and why one drug or chemical would affect similar cells differently, and why this sort of experimental manipulation may ...

Purchase this Solution


Free BrainMass Quizzes
How Well Do You Know Your Body?

This quiz will assess the different systems of the human body. It will examine everything from the organs to the cellular processes that occur.

Parts of the Brain

This quiz will test your knowledge on different areas of the brain.

Identifying Variables in Science Experiments, Part 2

Using sample experiments, test yourself to see if you can identify independent, dependent, and controlled variables. Identifying variables is key in understanding and developing experiments. The questions are biology related, but this can be applied to any area of science.

The Heart

This quiz test the understanding of the heart and some of its parts. It is important to understand how the heart functions and what makes it function.

Breastfeeding Basics

How much do you know about breastfeeding? Find out with this quiz!