Understanding Beer's Law
What is Beer's law? Use Beer's law to mathematically determine the unknown concentration values. How do the values derived from Beer's law compare to those derived from the graph?
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You usually use a colorimeter where red light from the LED light source will pass through the solution in question and strike a photocell. A higher concentration of the solution absorbs more light (and transmits less) than a solution of lower concentration. The colorimeter monitors the light received by the photocell as either an absorbance or a percent transmittance value.
You usually prepare at least five solutions of a known concentration (standard solutions). Each is transferred to a small, rectangular cuvette that is placed into the colorimeter. The amount of light that penetrates the solution and strikes the photocell is used to compute the absorbance of each solution. When a graph of absorbance versus concentration is plotted for ...
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Full explanation with solution graph. 474 words.