Understanding Beer's Law
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What is Beer's law? Use Beer's law to mathematically determine the unknown concentration values. How do the values derived from Beer's law compare to those derived from the graph?
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You usually use a colorimeter where red light from the LED light source will pass through the solution in question and strike a photocell. A higher concentration of the solution absorbs more light (and transmits less) than a solution of lower concentration. The colorimeter monitors the light received by the photocell as either an absorbance or a percent transmittance value.
You usually prepare at least five solutions of a known concentration (standard solutions). Each is transferred to a small, rectangular cuvette that is placed into the colorimeter. The amount of light that penetrates the solution and strikes the photocell is used to compute the absorbance of each solution. When a graph of absorbance versus concentration is plotted for ...
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