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# Spectrophotometric Measurements

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What are the advantages and disadvantages in using spectrophotometric measurements for the analysis of various compounds?

Explain Beer's and Lambert's laws and how are these important to spectrophotometric measurements?
How are spectrophotometric measurements used for the determinations of content of phenolic compounds in plants ? What could be possible problems involves in these (specific) procedures and what has been identified in the literature? What are some suggestions for the elimination of these problems?

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This solution offers detailed and logical answers to some questions related to spectrophotometric measurements, along with a relevant reference.

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A.

A spectrophotometer is an instrument that measures the amount of photons (the intensity of light) absorbed after it passes through sample solution. With a spectrophotometer, the amount of a known chemical substance (concentrations) can also be determined by measuring the intensity of light detected. Following are the advantages and disadvantages of spectrophotometric measurements:

The Lambert-Beer law is the linear relationship between absorbance and concentration of an absorbing species. The law is usually written as:
A = a( ) * l * c
where A is ...

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