The determination of iron in industrial waste stream is often carried out by reacting the water sample with o-phenanthroline to form a red-orange complex that absorbs at 510 nm. Using the data shown in the following table construct the standard calibration curve and determine the concentration of Fe in the waste stream. The cuvette or cell dimension is 1.00 cm.
ppm Fe Absorbance
CREATION OF A STANDARD CURVE AND DETERMINATION OF AN UNKNOWN SAMPLE
ABSORBANCE AND BEER'S LAW
Here is a straight forward approach to solving the problem. Please see the Excel file for the data work up. Nevertheless, you should practice doing it on your own so that you learn how.
Step 1: The first thing you do is transfer the data to an Excel file.
The solution gives the clear steps to apply concept of absorbency and Beer's Law to find the concentration. A standard curve is created to determine the unknown samples are determined.