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Reverse-Phase HPLC

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Choose any type of chemical analyte in a food product and please describe in detail how to analyze the analyte using a reverse-phase HPLC. Please include sample preparation, extraction and indicate what mobile phase, column, and detector were used.

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A brief discussion on using reverse phase HPLC to separate components in beverage is included.

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Caffeine along with sweeteners constitutes many beverages we drink today. A sample of a beverage can be taken and then degassed to remove any carbon dioxide at room temperature. The solution should be filtered and the filtrate used in further steps. As sweeteners are used to sweeten other foods as well like desserts and jams, a similar procedure can be used to separate these compounds.

For reverse phase HPLC, the stationary phase used can be SiO2 coated with long alkane ...

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