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Method Set-Up for a Chemical Separation

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Suggest a buffer and mobile phase composition to get the best separation given:
1. HPLC instrument
2. C18 reverse phase column
3. 1mM solution of each of the following compounds in Methanol
4. Mobile phase is methanol-buffer mixture

Compounds are 3 seperate groups:
1. Carboxylic Acids:
a. Benzoic acid
b. Phthalic acid
c. Toluic acid

2. Amino:
a. aniline
b. 4-aminotoluene
c. orhto-pheylenediamine

3.Amino Carboylic Acids:
a. 4-aminobenzoic acid
b. 2-aminobenzoic acid
c.4-amino-2-methylbenzoic acid

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Solution Summary

The methods set-up for a chemical separation are discussed. The solution is 225 words. The reverse phase columns are given.

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To answer this interesting question, I think you will need to remember how the chromatography process works. I think it is desirable to have the solute particles to have some time on the column and some time in the mobile phase.

Since you are using a reversed phase column, the mobile phase will be ...

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