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Biochemical and behavioural Pharmacology

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Hi, I have attached 2 calculations file. Using those calcuations, I am supposed to draw a log graph, However, I am totally clueless as to how I am supposed to proceed with it in regards to obtaining curves etc.

Therefore, If somebody could start me off and give me an indiaction or explanation as to how I am supposed to draw the graph, I would be grateful.


PS I know there is no log graph attached but if somebody could maybe draw a sample on word or other application it would be great.

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As a sample explanation, I drew the graph corresponding to the second table on "Calculations.jpg." This is the table that has handwritten underneath it, "Carbachol + 20 nM Atropine." You should be able to do the rest of the data yourself by examining the details below as well as in the attached Excel file.


1. Transfer the data into Excel by typing the concentration of stock solution into ...

Solution Summary

Biochemical and behavioural pharmacology is discussed. The process in regards to obtaining curves is determined.

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Experiment: Affinity for competitive antagonists

Experiment to determine the affinity for competitive antagonists at cholinoceptors in isolated tissues.

All the information is in the attached files. The first 3 files provide a description of what the experiment was about, how we carried it out, and theory etc.

What i want you to do is look at the attachments and the following:

INTRODUCTION: A brief indication of the aims of the experiment, a definition of competitive antagonism, and of the parameters investigated and an indication as to why it is important to be able to determine pA2, kA etc. Include an indication of which subtype of muscuranic receptor is present in the tissue and how it brings about its response when activated.

METHOD: A mention of the general methods (very brief) and anything specific which is not covered in the schedule (eg weight and sex of animals used)

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