Experiment to determine the affinity for competitive antagonists at cholinoceptors in isolated tissues.
What i want you to do is look at the attachments and the following:
INTRODUCTION: A brief indication of the aims of the experiment, a definition of competitive antagonism, and of the parameters investigated and an indication as to why it is important to be able to determine pA2, kA etc. Include an indication of which subtype of muscuranic receptor is present in the tissue and how it brings about its response when activated.
METHOD: A mention of the general methods (very brief) and anything specific which is not covered in the schedule (eg weight and sex of animals used)
Please see response attached. I hope this helps and take care.
Biochemical and behavioural Pharmacology
Experiment to determine the affinity for competitive antagonists at cholinoceptors in isolated tissues
All the information is in the attached files.
The first 3 files provide a description of what the experiment was about, how we carried it out, and theory etc.
If you have already completed this experiment, these types of things need to be worked out prior to the experiment. For example, how could you complete an experiment and not know the method that you used or the aim(s) of the experiment? Or, it this an experiment given to you as an assignment, and you have to delineate what the researchers actually did?
Either way, let's look at your findings and the questions posed below. I will glean your findings and see where it is that I can be helpful. First, though it is important to consider why experiments such as these are done. Basically, pharmacological studies use animals (e.g., guinea pigs, etc) to test their new drugs in the development and testing phases to determine the right concentrations for certain the desired effects. For example, for depression, the seretonin and norepinephrine ion receptor sites are implicated, so in the development of the SSRIs, a similar experiment would have been done in the developmental stages to test if the desired effects occurred (inhibition of seretonin receptor, etc.). The actual experimental procedures seems complicated because of the use of empirical values ...
Referring to the article, this solution assists in analyzing the introduction and methods by responding to the questions posed.