Chemistry: Catalase Sample Questions
Not what you're looking for?
We did the catalse test in class and I have been able to answer some questions but not all.
Questions:
1) Provide an example of reduction reaction performed by cells in which hydrogen is not added to the reduced compound. (I think it deals with hydrogen peroxide--> to water and oxygen)
2) Would a flase positive due to the reaction between the loop and hydrogen peroxide be due to a lack of specificy or sensitivity of the test system?
3) If a weakly catalase-positive organism itinitially gives a false-negative result and is not checked under the miicroscope, would the result be due to a lack of specificity or sensitivity of the test system?
4) Why is it advisable to perform this test on a known catalase-positive organism simultaneously with the organism you are checking?
5) What is the purpose of adding hydrogen peroxide to the uninoculated tube?
Purchase this Solution
Solution Preview
(1) Provide an example of reduction reaction performed by cells in which hydrogen is not added to the reduced compound.
You're correct. 2 H2O2 ----> 2 H2O + O2
(2) Would a false positive due to the reaction between the loop and hydrogen peroxide be due to a lack of specificity or sensitivity of the test system?
Response: Such a false positive reaction would be due to a lack of specificity in the test system. In other words, the test system is sensitive enough, the problem being that "other" things going on in the test system also gives a "positive" result. Therefore, the issue here has to do with a lack of specificity.
(3) If a weakly catalase-positive organism itinitially gives a false-negative result and ...
Purchase this Solution
Free BrainMass Quizzes
Breastfeeding Basics
How much do you know about breastfeeding? Find out with this quiz!
Parts of the Brain
This quiz will test your knowledge on different areas of the brain.
Birth 101
Do you know about childbirth? Find out with this quiz.
Vision and Oculomotor Control
This quiz will test the student's knowledge of the neural underpinnings of the visual system and its central pathways.
Understanding the Musculoskeletal system
Introduce and understand basic information how the skeletal system and muscular system work in close concert with one another. And how their interaction between muscle and bone, as they work together to allow us movement.