Explore BrainMass

Scientific Experiment Report

This content was STOLEN from BrainMass.com - View the original, and get the already-completed solution here!

Can you answer this problem for me in a good detailed form?

This is the experiment I have to experiment estimate some results and do a conclusion and discussion of the way in which the method may of reflected in my results can you:
Predict the results
Explain results you have predicted
Give sources of error for the method

How could the experiment could be modified to determine the optimum pH more accurately?
Limitations of the experiment and improvements

Cut squares of developed film measuring 0.5cm x 0,5cm as accurately as you can. Thirty squares should be sufficient for the experiment.
Measure 2cm2 of trypsin into a test tube and add 2cm2 of one of the buffer solutions at a particular pH. Do this twice more so you have three test tubes with a mixture at that pH.
Put them in a water bath at 37 degrees leave for 2 minutes.
Add a square of film to the mixture in each tube in the water bath and start the stop clock
Cheak each tube frequently for signs that the gelatine has been digested. You will see the black silver falling from the film and turning the mixture grey. Shake the tube each time check it. Record the time taken for digestion of the gelatine.
Repeat steps 2 to 6 using buffers at the other pHs. Remember 3 tubes per pH.

© BrainMass Inc. brainmass.com October 16, 2018, 4:05 pm ad1c9bdddf

Solution Preview

Large molecules like gelatin can be precipitated with trichloroacetic acid. Then the absorbance of the digested proteins can be measured at 280nm, and compared against the absorbance of a blank.

0.02 M Tris-HCl buffer, pH ...

Solution Summary

This posting provides an scientific report of an experiment in which the results predicted and sources of error and optimization of the method are discussed.

Similar Posting

Help with questions concerning Theory X and Y, the Hawthorne experiments, definition of post-bureaucratic organization, scientific management and organization theory

1. Which of the following is true about Theory X and Theory Y?
A. The Hawthorne experiments support Theory X and Theory Y.
B. Theory Y supports autonomy and Theory X challenges autonomy.
C. Theory Y is outdated due to current laws and regulations, which leaves Theory X as the only option.
D. Theory X is more cost effective than Theory Y.

2. What did the Hawthorne experiments report?
A. It reported on motivations and social responses of workers in an organization
B. It confirmed that positive reinforcement is most effective before noon for good sleepers.
C. It discussed the productivity of line workers versus productivity of the management team.
D. It reported that smoking does not correlate to arrival time to work.

3. Which of the following is an example of a post-bureaucratic organization?
A. Bureaucracy remains and the hierarchy expands
B. The removal of all senators from office
C. Bills become laws through the executive branch
D. A task force with diverse experts

4. What is scientific management?
A. It identifies the biological effects of management.
B. It is the systematic approach to using the most efficient methods with the least amount of resistance.
C. It is the organization and arrangement of formulas in the public sector.
D. It is a new form of government to retain environmental activists.

What is organization theory?
A. The study of how to relieve oneself from chaos
B. The study of management actions
C. The study of group and individual behavior within organizational arrangements
D. The study of organized groupthink

View Full Posting Details