Purchase Solution

Flow cytometry

Not what you're looking for?

Ask Custom Question

Hi. Can someone please check my answers to the following multi-part question, and explain the questions I couldn't answer or that I got wrong?

This is an 11-part question (!), but I think it looks worse than it is. It's 60% of a 1-hour test, so I don't think it should take more than an hour to go through, at most.

For the question, please go to the following website: http://tutor.lscf.ucsb.edu/instdev/sears/immunology/

Click on prior examinations; password: gauchos

Go to winter 2003 midterm, question #2. (My professor gave out previous years' exam questions, but not answers.)

One thing I'm particularly concerned about in my answers is that I didn't take into account any differences between spleen lymphocytes and thymus lymphocytes, because I wasn't sure what those differences might be.

Here are my answers:

A. T cytotoxic cells -- because these are CD8+,CD4- lymphocytes.

B. T helper cells -- because these are CD4+,CD8- lymphocytes.

C. These are CD4-,CD8- (double negative) lymphocytes, so they could be either B cells or NK cells, both of which are lymphocytes that lack CD4 and CD8 markers.

D. These are TCR(alpha,beta)+, CD8- lymphocytes, so they are probably T helper cells. They could also be NK cells (which would have an alpha beta T cell receptor, and would lack a CD8 marker.)

E. TCR(alpha, beta)+, CD8+ lymphocytes = T cytotoxic cells.

F. TCR(alpha,beta)-, CD8- lymphocytes = B cells. The only lymphocytes that lack T cell receptors are B lymphocytes.

G. I'm not sure about this one. Could they be T cytoxic cells that have TCR(gamma, delta) instead of TCR(alpha, beta)?

H. I'm *guessing* that it's the lower left quadrant based on the fact that those cells are CD4-,CD8- and hence can't function as T helper cells or T cytotoxic cells. But couldn't those cells be B cells or NK cells, rather than functionally immature cells?

I. I have no idea. Please help!

J. I would *guess* that the answer is T helper cells since there seem to be relatively small changes in their populations from the normal mice to the knockout mice. Or is a more accurate answer "all CD8- cells, including T helper cells, B cells, and NK cells"?

K. I have no idea. Please help.

Thank you!!

Purchase this Solution

Purchase this Solution

Free BrainMass Quizzes
Understanding the Musculoskeletal system

Introduce and understand basic information how the skeletal system and muscular system work in close concert with one another. And how their interaction between muscle and bone, as they work together to allow us movement.

Parts of the Brain

This quiz will test your knowledge on different areas of the brain.

BioChemistry Basics

This Quiz will test your knowledge of the amino acids used in biological systems

Breastfeeding Basics

How much do you know about breastfeeding? Find out with this quiz!

Light and Sight Vocabulary

This quiz introduces basic definitions of vocabulary related to light and how human eyes. This information is important for an understanding of sight.