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    Predicting the correct translation frame

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    Your colleague has an EST fragment from E. coli with the following sequence: AAGUCAUUAUUUUCG. Assuming this is the coding strand, can you help her to identify the most likely translation frame?

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    Solution Preview

    If you use this website: http://molbiol.ru/eng/scripts/01_13.html and input your sequence you should find your protein translation.

    In your case, you want to search all six reading frames. That means you translate the triplet codons of your sequence starting from either the forward direction or reverse direction as shown below. Then you start translating from either the first (+1 ...

    Solution Summary

    This question deals with predicting the translation frame of a sequence. As with all DNA sequence related questions, you need to use a web-based search engine to manipulate and analyze a sequence. This solution provides a website and shows you how to analyze your sequence. The question is answered with an explanation as to how the solution was rendered.