Your colleague has an EST fragment from E. coli with the following sequence: AAGUCAUUAUUUUCG. Assuming this is the coding strand, can you help her to identify the most likely translation frame?© BrainMass Inc. brainmass.com October 25, 2018, 6:35 am ad1c9bdddf
If you use this website: http://molbiol.ru/eng/scripts/01_13.html and input your sequence you should find your protein translation.
In your case, you want to search all six reading frames. That means you translate the triplet codons of your sequence starting from either the forward direction or reverse direction as shown below. Then you start translating from either the first (+1 ...
This question deals with predicting the translation frame of a sequence. As with all DNA sequence related questions, you need to use a web-based search engine to manipulate and analyze a sequence. This solution provides a website and shows you how to analyze your sequence. The question is answered with an explanation as to how the solution was rendered.
Translation in Prokaryotes and Eukaryotes
1. Using the following mRNA sequence: 5'-ACAAUCCUGCAUGCCGAUGCGAGCCGAAUAAGCACGUGUU-3'
complete the following:
a. Indicate the start codon.
b. Indicate the stop codon.
c. Give the proper sequence of amino acids if this sequence was translated.
2. For each of the following, give a brief description:
a. initiation complex
b. Shine-Delgarno sequence
c. activation and charging of an amino acid
3. Describe what is meant by the degeneracy of the genetic code, and how this is dealt with in the cell. Include in your answer isoaccepting tRNAs and the wooble hypothesis.
a. Describe in detail the molecular mechanisms involved in termination of translation.
b. What is the proposed mechanism of action of some antibiotics? Give an example.
5. Describe the following:
a. What is meant by the "almost universal genetic code"?
b. How do we know that there is no punctuation in the genetic code?
c. How can we predict the number of genes in an organism's genome?
d. What is meant by the C-value paradox?