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Gene insertion through transgenic technology

In order to study the function of a gene you have cloned in the mouse, you decide to inactivate it using transgenic technology. Using recombinant DNA techniques, you construct a modified version of the cloned gene by introducing an antibiotic resistance gene into middle of it.

Into what kind of cells you will need to introduce this modified version of the gene?
If instead of modifying an existing gene in a mouse, you wished to add a gene from a jellyfish with no counterpart in the mouse genome, what general method could you use?

Solution Preview

Modified version of gene can be introduced either of two ways. DNA can be integrated in a random fashion by injecting it into the pronucleus of a fertilized ovum. In this case, the DNA can integrate anywhere in the genome, and multiple copies often ...

Solution Summary

Modified version of gene can be introduced either of two ways. DNA can be integrated in a random fashion by injecting it into the pronucleus of a fertilized ovum. Or, introducing the DNA into embryonic stem (ES) cells and selecting for cells in which the DNA has undergone homologous recombination.

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