Would you help me with the following questions?
a. In an experiment, [A]=6 mM, and the initial velocity, V0, was 480 nM min -1. What was the [Et] used in the experiment?
b. In another experiment, [Et] = 0.5 uM, and the measured V0 = 5 uM
min-1. What was the [A] used in the experiment?
For these questions it is important to keep your units all worked out.
The two equations to have in mind:
V0 = (Vmax . [S])/(Km + [S])
Vmax = Kcat [E]tot
a) V0 = 480 nM min-1= 480 x ...
This solution helps with problems about enzymes.