The order of nucleotides is critical in determining the amino acid that is encoded.
What do the following trinucleotides code for: AAC, ACA, CAA, CAC?
What would be the sequence of a protein consisting of a repeat of each of these trinucleotides? If the sequence was read in a different reading frame, how would this affect the protein product?
AAC codes for Asn
ACA codes for Thr
CAA codes for Gln
CAC codes for His
Therefore the first sequence:
encodes Asn-Asn-Asn etc. in the first reading frame
and Thr-Thr-Thr etc. in the second ...
This solution explains how the order of the nucleotides in a trinucleotide RNA codon affects the amino acid that is encoded. It gives examples of proteins encoded by repeating codons, and how the reading frame affects the protein produced.