Genetics-From genes to protein, mutations.
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Transcribe and translate EACH of the three following DNA gene sequences. Turn these in using the graphic organizer. The letters represent the DNA N-base sequences of the genes.
The first is the original gene. The next two are mutations of the original. Note that the changes (mutations) are shown in parenthesis.
Original Gene sequence 3'-T A C C C T T T A G T A G C C A C T-5
Mutated gene sequence 1 3'-T A C (G) C T T T A G T A G C C A (T) T-5'
Mutated gene sequence 2 3'-T A (A) C C T T T A (C) T A G (G) C A C T-5'
What is the significance of the first and last codons of an mRNA transcript?
What meaning do these mRNA codons have for protein synthesis?
Did the mutations result in a change in the final proteins? If so, describe the change.
In general, why might a change in amino acid sequence affect protein function?
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Solution Summary
Transcription and translation are the dogma of molecular biology. DNA is transcibed into mRNA by DNA Polymerase. mRNA is then translated into protein by the process of translation. These are the process of DNA synthesis.
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Part I
Original DNA Strand:
3'-T A C C C T T T A G T A G C C A C T-5'
Transcription (base sequence of RNA):
5'-AUGGGAAAUCAUCGGUGA-3'
Translation (amino acid sequence):
Met-Gly-Asn-His-Arg-Stop
Mutated gene sequence one:
3'-T A C G C T T T A G T A G C C A T T-5'
Transcription (base sequence of RNA):
5'-AUGCGAAAUCAUCGGUAA-3'
Translation (amino acid sequence):
Met-Arg-Asn-His-Arg-Stop
Mutated gene sequence two:
3'-T A A C C T T T A C T A G G C A C ...
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