Identify Protein From Nucleotide Sequence Using BLAST

Related BrainMass Solutions

• Use of BLAST online database to search for protein identity

  Using the results answering the following: (a) Why does an amino acid query sequence leads to a higher similarity than a nucleotide query sequence?

• Open reading frame & deciphering the target protein of interest

  This solution explains how to identify an open reading frame given a DNA sequence in order to determine the protein sequence and ultimately its identify using online databases such as those of the NCBI and Expasy.

• Predicting the correct translation frame

  A quick search of the BLAST website (http://blast.ncbi.nlm.nih.gov/Blast.cgi?CMD=Web&PAGE_TYPE=BlastHome) using the 15 base nucleotide sequence provided above does not reveal any matches with known ESTs to date.

• Analysis of a Gene Sequence

  You can obtain NM_007527 sequence by searching Nucleotide for NM_007527 from the the NCBI website. What is the human ortholog of NM_007527?

• Effect of nucleotide substitution mutation on protein

  This can be better explained using the different amino acid codons.

• Bioinformatics questions

  We assume our sequence (gene or protein) is the correct one, sequentially and from the right organism e. We assume we are correctly performing the alignment f.

• Calculating numbers of RNA nucleotides from amino acids.

  Thanks for using BrainMass. Method of back calculating from a given number of amino acids (which make up a protein) to find the number of nucleotides in an mRNA or DNA sequence.

• DNA Sequence Analysis

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• Which Point Mutation is Catastrophic to Protein Function?

  of the coding sequence A point mutation, or substitution, is a type of mutation that causes the replacement of a single base nucleotide with another nucleotide.