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Descriptive Techniques used for Biological Changes

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For the first question, I thought PCR might have something to do with it, but it seems to restrictive. I can't think of any other descriptive techniques. can anyone suggest any?

For question 2, it look simple but when a deletion occurs does it happen to the top and bottom strand? I was just wondering because that changes the whole complex of DNA. if it only happens in one strand then some strands will be shorter than others.

1. A biologist discovers an obscure bacterium with an enzyme that catalyzes the breakdown of a toxic byproduct of computer-chip manufacturing. It has real commercial potential, but it is made in very small quantities, and the bacterium cannot be grown very easily. How should she begin to think of realizing the commercial potential of her discovery?

2. Starting with the segment of a double-stranded DNA molecule shown, grouped in codons, give the sequence of the molecule resulting from each of the mutations indicated below. Show 3´ and 5´ ends, and write mutant base pairs in lowercase letters. Classify these mutations with respect to their molecular outcomes. (Position numbers below refer to the top strand.)

(a) A transition at base pair position 5
(b) A transversion at base pair position 5
(c) A deletion of 2 base pairs at positions 4 and 5, respectively
(d) An insertion of 3 base pairs between base pairs 5 and 6
(e) An inversion of the 3 base pairs beginning at position

See attached file for full problem description.

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Solution Summary

The expert examines descriptive techniques used for biological changes. The whole complex of DNA are given.

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Your right. For question 1 PCR is too restrictive. There is another very common and important molecular biology technique that will help. And I gave you a start on the 2nd question. All mutations in DNA occur in both strands to keep them the same length. (See solution file below).

1. A biologist discovers an obscure bacterium with an enzyme that catalyzes the breakdown of a toxic byproduct of computer-chip manufacturing. It has real commercial potential, but it is made in very small quantities, and the bacterium cannot be grown very easily. How should she begin to think of realizing the commercial potential of her discovery?
Think about an experimental system that you can use to make lots of a protein (enzymes are proteins). PCR only ...

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