What is the difference between reproductive and therapeutic cloning? Name one limitation for each technique. Make sure to differentiate between pluripotent and totipotent embryonic stem cells as well as adult and embryonic stem cells.© BrainMass Inc. brainmass.com September 18, 2018, 7:48 pm ad1c9bdddf - https://brainmass.com/biology/cloning/main-differences-between-reproductive-and-therapeutic-cloning-3222
The source of pluripotent embryonic stem cells is the blastocyst inner cell mass (ICM) and should not be confused with adult stem cells (Non Embryonic Stem Cells), which can come from bone marrow, muscle, cord blood/placenta, blood and even fat tissues. Adult stem cells have a limited differentiation potential since they can only differentiate into specific/defined cell types. For example the pluripotential hematopoietic stem cell gives rise to erythrocytes, neutrophils, basophils, eosinophils, platelets, mast cells, monocytes, tissue macrophages, osteoclasts, and the T and B lymphocytes stem cells. This is a large cell number compared to other adult stem cells, however a pluripotent embryonic stem cell can give rise to even larger cell number including anything from three germ layers: ectoderm, mesoderm and endoderm.
Pluripotent embryonic stem cells can differentiate into almost anything except placenta.
Totipotent cells can differentiate into anything including placenta.
Involves extracting diploid somatic cell nucleus from adult cells ...