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Progression of fluorescent proteins

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Give three specific examples of a property that was improved by mutation of the DsRed. These must be three different types of improvements and for each example you should refer to a different mutant FP.

So for example, DsRed --> mRFP --> mGrape1 / mStrawberry etc.

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Solution Preview

Making a better fluorescent protein is usually achieved by stepwise point mutations that improve the over all effect of the protein. In your example dsRED was the initial form and the subsequent versions (RFP, Grape etc) are improvements on the properties ideal for a fluorescent fusion ...

Solution Summary

Discussion of the differences between fluorescent proteins and the properties in creating these proteins.

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