Purchase Solution

Lactate Dehydrogenase and Disulfiram (Antabuse)

Not what you're looking for?

Ask Custom Question

Questions
1 - The enzyme loctate dehydrogenase (LDH) is present in all tissues as a catalyst of anaerobic glycolysis. It has 5 isoforms, describe these isoforms and how they can be used in differential diagnostic tests.
2 - Disulfiram (Antabuse) is a drug used in the treatment of alcoholism. What enzyme reaction does this drug affect and why does it leave the patient feeling ill?

The above questions only need to be about half a page for each question.

Purchase this Solution
Solution Summary

Half a page is given for each question and specifics are given regarding lactate dehydrogenase and disulfiram.

Solution Preview

1 - The enzyme lactate dehydrogenase (LDH) is present in all tissues as a catalyst of anaerobic glycolysis. It has 5 isoforms, describe these isoforms and how they can be used in differential diagnostic tests.

Lactate dehydrogenase [LDH] was the first enzyme found to have isozymes separable by electrophoresis. These isozymes differ in the polypeptide chain composition and sequence although they share evolutionary origin. They also differ in the metabolic patterns in the different organs where they are found.

LDH molecule has 4 subunits and they are recognized as H and M based on their electrophoretic properties.

LDH-1 [4H] found in the heart
LDH-2 [3H1M] - reticuloendothelial system
LDH-3 [2H2M] - in lungs
LDH-4 [1H3M] - in kidneys
LDH-5 [4M] - in liver ...

Purchase this Solution
Free BrainMass Quizzes
Biochemistry Basics

A refresher quiz to test your knowledge of basics concepts of biochemistry.

Feeding Babies

Do you know the science behind feeding babies? Test your knowledge with this quiz.

Breast Milk and Breastfeeding

How much do you know about breast milk and breastfeeding? Double check your knowledge level with this quiz!

Basic Immunology Quiz

Intro to immuno quiz. Covers the basics of immunology and recognition of foreign substances by the body.

Birth 101

Do you know about childbirth? Find out with this quiz.

View More Free Quizzes
Related BrainMass Solutions

  • Reaction process for Lactate Dehydrogenase catayzed

    go if NAD^(+), NADH, pyruvate, and lactate were mixed, all at 1 M concentrations, in the presence of lactate dehydrogenase at pH 7; (b) calculate ΔG'° for this reaction; (c) calculate Keq' for this reaction.

  • Questions Based on the Action of Pyruvate

    Excess pyruvate is converted to lactate, which accumulates in tissues and in the blood, causing lactic acidosis. a.

  • Medications used for substance abusers.

    Buprendorphine, Suboxone and Naltrexone are also used to help individuals reduce their use of heroine. Disulfiram (Antabuse) is a medication used on alcoholics. The medication is used to induce nausea when alcohol is consumed.

  • Anaerobic Cellular Respiration: Pyruvate Conversion Products

    In addition, glucose can be synthesized from noncarbohydrate precursors, such as pyruvate and lactic acid, in the process of gluconeogenesis. As an aside, Pyruvate can be reduced to lactate by lactate dehydrogenase to regenerate NAD+.

  • LDH isoforms and relative percentages in different tissues

    Explain Lactate dehydrogenase (LDH) is an enzyme (EC 1.1.1.27) present in a wide variety of organisms, including plants and animals. It catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+.

  • Concept map on metabolism

    Under anaerobic conditions and in erythrocytes under aerobic conditions, pyruvate is converted to lactate by the enzyme lactate dehydrogenase (LDH), and the lactate is transported out of the cell into the circulation.

  • Gibb's free energy change and equilibrium constant

    The following reaction, which takes place just before the citric acid cycle, is catalyzed by the enzyme lactate dehydrogenase CH3COCOO- + NADH + H+ <----> CH3CH(OH)COO- + NAD+ From the data listed in Table 7.2, calculate the value of Gibb's free

  • NMR Spectrum and 13C Label

    Once again, this is accomplished by a single reaction catalyzed by lactate dehydrogenase. Pyruvate + NADN + H+ -----> lactate + NAD+ This is a typical redox reaction.

  • Isozymes Examination

    A typical example is shown in the article from the website given below: http://medical-dictionary.thefreedictionary.com/Lactate+Dehydrogenase+Isoenzymes+Test Copy and paste the site in your browser for full details of what an elevated level of one

View More Related Solutions