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Lactate Dehydrogenase and Disulfiram (Antabuse)

Questions
1 - The enzyme loctate dehydrogenase (LDH) is present in all tissues as a catalyst of anaerobic glycolysis. It has 5 isoforms, describe these isoforms and how they can be used in differential diagnostic tests.
2 - Disulfiram (Antabuse) is a drug used in the treatment of alcoholism. What enzyme reaction does this drug affect and why does it leave the patient feeling ill?

The above questions only need to be about half a page for each question.

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Solution Preview

1 - The enzyme lactate dehydrogenase (LDH) is present in all tissues as a catalyst of anaerobic glycolysis. It has 5 isoforms, describe these isoforms and how they can be used in differential diagnostic tests.

Lactate dehydrogenase [LDH] was the first enzyme found to have isozymes separable by electrophoresis. These isozymes differ in the polypeptide chain composition and sequence although they share evolutionary origin. They also differ in the metabolic patterns in the different organs where they are found.

LDH molecule has 4 subunits and they are recognized as H and M based on their electrophoretic properties.

LDH-1 [4H] found in the heart
LDH-2 [3H1M] - reticuloendothelial system
LDH-3 [2H2M] - in lungs
LDH-4 [1H3M] - in kidneys
LDH-5 [4M] - in liver and ...

Solution Summary

Half a page is given for each question and specifics are given.

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