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LDH isoforms and relative percentages in different tissues

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1. Please explain the data attached and why the percentages in each are as so.

2. Which sample has the highest and lowest amount of polypeptide chain H? Explain

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Solution Preview

Lactate dehydrogenase (LDH) is an enzyme (EC present in a wide variety of organisms, including plants and animals. It catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. As it can also catalyze the oxidation of hydroxybutyrate, it is occasionally called Hydroxybutyrate Dehydrogenase (HBD).

Enzyme isoforms
Lactate dehydrogenase (LDH) is composed of 4 subunits (a tetramer), produced from combinations of 2 gene products (Ldhm, Ldhh).

- LDH-1 (4H) - in the heart
- LDH-2 (3H1M) - in the reticuloendothelial system
- LDH-3 (2H2M) - in the lungs
- LDH-4 (1H3M) - in the kidneys
- ...

Solution Summary

This solution provides detailed information on LDH, its importance and different isoforms of LDH present in tissues. Then it provides the relative percentages of these isoforms in different tissues and answers a few important questions.