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GRE Subject Question

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How many different primary sequences are possible for a pentapeptide made from naturally occurring amino acids?

Considering that most proteins are composed of one hundred or more what conclusions can be drawn from the calculation?

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Considering that there are 20 naturally occuring amino acids, there could be any 1 of 20 in the first position. Then, there could be any 1 of 20 in the second position. That means ...

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BIOCHEM GRE Subject Question

You have been investigating a membrane protein that appears to have seven 24 residue transmembrane Alpha helices. Assume that each helix is perpendicular to the plane of the membrane and goes exactly from side of the membrane to the other. Also assume that each helix is linked to the next by a loop with a minimun of 12 amino acid residues.

A) From typical helix parameters and the information given, estimate the thickness of the membrane?

B) What is the minimum number of residues in this protein?

C) Using the result of B) estimate the molecular weight of this protein?

D) How many mainchain H-bonds are in each helix?

E) What types of amino acids residues do you expect to make up the bulk of each transmembrane helix?

F) What types of amino acids residues might you expect to find at the ends of each transmembrane helix?

G) Are the NH2-terminus and COOH terminus on the same side or opposite sides of the membrane?

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