Chymotrypsin
Not what you're looking for?
Chymotrypsin was able to hydrolyse the substrate N-benzoyl-L-tyrosine ethyl ester (BTEE) but not able to hydrolyse the substrate p-toluene sulphonyyl-L-arginine methyl ester (TAME).
However the enzyme trypsin was able to hydrolyse both TAME and BTEE substrates, and I was wondering why? And what makes chymotrypsin so much more specific?
Purchase this Solution
Solution Summary
In this solution we explain why the enzyme trypsin is able to hydrolyse both TAME and BTEE substrates. Additionally, it compares properties of chymotrypsin to trypsin. The solution also includes one reference source for further explanation and research of the subject.
Solution Preview
Thank you for using BrainMass.com. I hope that this solution helps you with your assignment.
It is not really that chymotrypsin is so much more specific, but that p-toluene sulphonyyl-L-arginine methyl ester (TAME) has functional groups that are inhibitors of chymotrypsin and not trypsin. Compare the following structures for TAME and tosyl phenylalanyl chloromethyl ketone (TPCK). TPCK is a ...
Purchase this Solution
Free BrainMass Quizzes
Bacterial Genetics
This quiz test your knowledge of the genetics of bacteria.
Feeding Babies
Do you know the science behind feeding babies? Test your knowledge with this quiz.
Basic Immunology Quiz
Intro to immuno quiz. Covers the basics of immunology and recognition of foreign substances by the body.
Identifying Variables in Science Experiments, Part 2
Using sample experiments, test yourself to see if you can identify independent, dependent, and controlled variables. Identifying variables is key in understanding and developing experiments. The questions are biology related, but this can be applied to any area of science.
Biochemistry Basics
A refresher quiz to test your knowledge of basics concepts of biochemistry.