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Solve: Octapeptide Protein

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You have isolated and purified a a small octapeptide protein and want to determine its primary structure. Deduce the sequence from the following experimental results.

A) Complete amino acid analysis, corrected for amino acid hydrolysis loss yielded the following composition (Arg, Glu, Lys, Met, Ser, Thr, Trp, Tyr).

B) Reduction with Dansyl Chloride followed by acid hyrolysis and separation yielded the following dansylated compound:

Dansyl Chloride with an attached R group of (CH2OH)

C) Chymotrypsin yielded three fragments, a pentapeptide, a dipeptide containing Tyr, and the single amino acid Glu.

D) Reaction of the original polypeptide with CNBr produced two tetrapeptides. Reaction of one of these tetrapeptides with Edmans reagent followed by hydrolysisyieled a PTH derivative with a (CH2)4-NH3

E) Trypsin digestion of the original material resulted in the production of two tripeptides and one dipeptide.

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Solution Preview

You have isolated and purified a a small octapeptide protein and want to determine its primary structure. Deduce the sequence from the following experimental results.

A) Complete amino acid analysis, corrected for amino acid hydrolysis loss yielded the following composition (Arg, Glu, Lys, Met, Ser, Thr, Trp, Tyr)

B) Reduction with Dansyl Chloride followed by acid hyrolysis and separation yielded the following dansylated compound.:

Dansyl Chloride with an attached R group of ...

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