Purchase Solution

Solve: Octapeptide Protein

Not what you're looking for?

Ask Custom Question

You have isolated and purified a a small octapeptide protein and want to determine its primary structure. Deduce the sequence from the following experimental results.

A) Complete amino acid analysis, corrected for amino acid hydrolysis loss yielded the following composition (Arg, Glu, Lys, Met, Ser, Thr, Trp, Tyr).

B) Reduction with Dansyl Chloride followed by acid hyrolysis and separation yielded the following dansylated compound:

Dansyl Chloride with an attached R group of (CH2OH)

C) Chymotrypsin yielded three fragments, a pentapeptide, a dipeptide containing Tyr, and the single amino acid Glu.

D) Reaction of the original polypeptide with CNBr produced two tetrapeptides. Reaction of one of these tetrapeptides with Edmans reagent followed by hydrolysisyieled a PTH derivative with a (CH2)4-NH3

E) Trypsin digestion of the original material resulted in the production of two tripeptides and one dipeptide.

Purchase this Solution
Solution Summary

The solution is given as approximately 200 words of explanation.

Solution Preview

You have isolated and purified a a small octapeptide protein and want to determine its primary structure. Deduce the sequence from the following experimental results.

A) Complete amino acid analysis, corrected for amino acid hydrolysis loss yielded the following composition (Arg, Glu, Lys, Met, Ser, Thr, Trp, Tyr)

B) Reduction with Dansyl Chloride followed by acid hyrolysis and separation yielded the following dansylated compound.:

Dansyl Chloride with an attached R group of ...

Purchase this Solution
Free BrainMass Quizzes
Basic Concepts in Neuroscience

This quiz provides a review of the basic concepts in neuroscience.

Basic Immunology Quiz

Intro to immuno quiz. Covers the basics of immunology and recognition of foreign substances by the body.

Parts of the Brain

This quiz will test your knowledge on different areas of the brain.

Light and Sight Vocabulary

This quiz introduces basic definitions of vocabulary related to light and how human eyes. This information is important for an understanding of sight.

The Plant Body

This quiz will test your knowledge of the anatomy of a common plant.

View More Free Quizzes
Related BrainMass Solutions

  • Determining mino acid sequences of peptide and octapeptide

    The first question is a step-by-step detail of how to solve the problem, while the second allows room for the student to use learned knowledge from the first question to complete it. Hints are provided in order to solve these problems in the future.

  • Peptide Sequencing

    GRE subject Question ________________________________________ You have isolated and purified a a small octapeptide protein and want to determine its primary structure. Deduce the sequence from the following experimental results.

  • Matching structures to descriptions

    an octapeptide with a C-terminal valine. b. an amino acid in its zqitterionic form. c. a polypeptide which gives four fragments on treatment with chymotrypsin d. the product of an edman degradation e. a carboxyl-protected amino acid. f.

  • Solving Systems of Equations using a Matrix Method

    Write an equation for the total amount of protein. C. Determine the augmented matrix that represents the previous two equations. D. Solve for the previous matrix. Show all work to receive full credit. E.

  • 6 Algebra Problems: Word Problems, Systems of Equations and Inequalities

    Soybean meal is 12% protein: cornmeal is 6% protein. How many pound of each should be mixed together in order to get 240-lb mixture that is 7% protein? How many pounds of the cornmeal should be in the mixture?

  • Algebra - Inequalities

    190347 Solving Inequalites - Soybean meal is 12% protein, cornmeal is 6% protein. How many pounds of each should be mixed together in order to get 240lbs mixture. ... 6. Soybean meal is 12% protein, cornmeal is 6% protein.

  • Solve by the Substitution Method

    179705 Solve by the Substitution Method 1) Solve by the substitution method 7x+5y=-12 -8x+y=54 What is the solution of the system? ( use an ordered pair. Use N if there is no solution) 2) Soybean meal is 18% protein; cornmeal is 9% protein.

  • Assignment - MTH 212 Unit 3 (B)

    Solve the system of equations using matrices. Show all work. Carbohydrates Protein Peanut Butter 2 6 Oats 5 1 A. Write an equation for the total amount of carbohydrates. B. Write an equation for the total amount of protein.

  • Using Matrices to Represent Information

    Help them solve the following problems. 4. The meteorologists at the National Interagency Fire Center had pizza delivered to their operations center. Their lunch consisted of pizza, milk and gelatin.

View More Related Solutions