Various Types of Microscopes and Their Uses
What type of microscope (Dissecting Microscope, Compound Light Microscope, TEM or SEM) you would use to examine the following specimens and explain WHY the choice of microscope is appropriate:
a) human tooth
b) human blood cell:
c) mitochondrial membrane folding
d) pollen grain surface structure
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SOLUTION This solution is FREE courtesy of BrainMass!
a) Human tooth: Dissecting Microscope
The image that appears in a Dissecting Microscope is three dimensional. It is used for dissection to get a better look at the larger specimen. One cannot see individual cells because it has a low magnification but that is not required here.
b) Human blood cell: Compound Light Microscope
Compound Light microscope is the most commonly used microscope. One can view individual cells, even living ones through this microscope. It has a high magnification that is sufficient for viewing human blood cell.
c) Mitochondrial membrane folding: TEM
Examining mitochondrial membrane folding requires 10,000x plus magnification which is just not possible using Light Microscopes. Hence TEM (Transmission Electron Microscope ) is used which provides the required magnification. TEM has a high magnification and high resolution.
d) Pollen grain surface structure: SEM
The surface structure is examined using the scanning action of electrons. Scanning Electron Microscope (SEM) is used for this purpose. SEM use electron illumination. The image is seen in 3-D. It has high magnification and high resolution. The specimen is coated in gold and the electrons bounce off to give an exterior view of the specimen.
https://brainmass.com/biology/cell-and-molecular-biology/various-types-microscopes-uses-4745