1. What type of amino acid side chains would you expect to find folded on the interior of a globular protein? What kind of experimental evidence supports this claim?
2. Which amino acid lacks a stereoisomer and which has more than one asymmetric carbon?
3. What property of the DNA double helix allows us to say that the recognition site for a restriction endonuclease like BamHI (GGATCC) is a structural palindrom?
4. What general kind of quarternary structure do we expect to find for the restriction endonuclease PstI, which also recognizes a palindromic sructure?
1) Globular proteins have a tertiary structure that is compactly folded and coiled.
First, to backtrack: Primary structure refers to the amino acid sequence, which completely determines all other structural features of a protein. This is due to the distinct chemical properties of the amino acid side chains - details which I will leave for your text. each type of protein has particular secondary structural elements; in globular proteins this is mostly alpha-helices. The amino acid residues found in alpha helices are alanine, aspartic acid, glutamic acid, isoleucine, and leucine. In the interior of a ...
Type II and Type I Restriction Endonucleases. ... Before answering, we need to understand the difference between type I and type II restriction endonucleases. ...
... a. Restriction Enzymes: Restriction endonucleases are enzymes, isolated primarily from prokaryotes, that recognize and cleave double-stranded DNA within ...
... The correct answer is B. The steps are below: 1. Restriction endonucleases are used to cut high-molecular-weight DNA strands into smaller fragments. ...
... beyond the hypothetical 10 tg of nuclear DNAs were digested with excess restriction (XhoI, BglII, StuI), and run on 1% agarose endonucleases hairpin structure ...
... In the Southern blot technique (110) DNA is digested w ith restriction endonucleases that specifically cleave at their individual recognition s equences. ...
... It allows one to predict sites of interaction with various transcription factors or endonucleases. For example, the restriction endonuclease BamHI recognizes ...
