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Titration of Sulfhydryl Groups on an Enzyme

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Many enzymes are inhibited irreversibly by heavy metal ions which react with essential sulfhydryl groups to form mercaptides. The affinity of silver ions (Ag+) for sulfhydryl groups is so great that it can be used to titrate -SH groups quantitatively. To a 10 ml of solution containing 10mg/ml pure enzyme was added just enough AgNO3 to completely inactivate the enzyme. A total of 0.342 μmol of AgNO3 was required. (a) Calculate the minimum molecular weight of the enzyme. (b)Why can you only calculate the minimum molecular weight from these data?

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The minimum molecular weight of an enzyme is calculatd in detail.

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Many enzymes are inhibited irreversibly by heavy metal ions which react with essential sulfhydryl groups to form mercaptides. The affinity of silver ions (Ag+) for sulfhydryl groups is so great that it can be used to titrate -SH groups quantitatively. To a 10 ml of solution containing 10mg/ml pure enzyme was added just enough AgNO3 to completely inactivate the ...

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