I have a particular strain of mouse and want to determine the DNA sequence of a specific region of one gene(and here we can assume the DNA to be tested
comes from one mouse and is NOT expected to be heterozygous at the locus tested i.e. assume uniform DNA content). I look at the known DNA sequence in that region (for normal mice) and choose two PCR primers to make. I expect to see a band of 1.6kb after PCR amplification but in fact I also see a fairly strong band of 0.9kb.
I need 3 ways that i could proceed with my sequencing objective(these can include materials and further experiments) explaining why each is likely to work.© BrainMass Inc. brainmass.com March 21, 2019, 6:54 pm ad1c9bdddf
This job explains mouse dna sequencing methods and explanations.