Purchase Solution

Mouse DNA Sequencing Methods and Explanations

Not what you're looking for?

Ask Custom Question

I have a particular strain of mouse and want to determine the DNA sequence of a specific region of one gene(and here we can assume the DNA to be tested
comes from one mouse and is NOT expected to be heterozygous at the locus tested i.e. assume uniform DNA content). I look at the known DNA sequence in that region (for normal mice) and choose two PCR primers to make. I expect to see a band of 1.6kb after PCR amplification but in fact I also see a fairly strong band of 0.9kb.

I need 3 ways that i could proceed with my sequencing objective(these can include materials and further experiments) explaining why each is likely to work.

Purchase this Solution

Solution Summary

This job explains mouse dna sequencing methods and explanations.

Purchase this Solution


Free BrainMass Quizzes
Vision and Oculomotor Control

This quiz will test the student's knowledge of the neural underpinnings of the visual system and its central pathways.

Identifying Variables in Science Experiments, Part 2

Using sample experiments, test yourself to see if you can identify independent, dependent, and controlled variables. Identifying variables is key in understanding and developing experiments. The questions are biology related, but this can be applied to any area of science.

Parts of the Brain

This quiz will test your knowledge on different areas of the brain.

Birth 101

Do you know about childbirth? Find out with this quiz.

Light and Sight Vocabulary

This quiz introduces basic definitions of vocabulary related to light and how human eyes. This information is important for an understanding of sight.