If we are given a mixed broth tube with 2 unknowns, how will you isolate it? How will you know when it's isolated?
Is it fair to say, that "Using sterile technique, I would preform the streak plate method. Once I can see, the 2 separate colonies in quadrant 4 of streak plate, I would put one of each colony into their own agar slant tube. I would know that they are isolated because you'll be able to see the difference on the streak plate but could also confirm through staining such as a gram stain.© BrainMass Inc. brainmass.com October 24, 2018, 7:49 pm ad1c9bdddf
It is very common to have a mixed population of bacteria growing in suspension. The easiest way to separate out these populations is to plate a small amount of the liquide on an agar plate. The sample is usually placed in one quadrant and then using a sterilized loop the liquide is streaked across each quadrant individually ...
Brief explanation regarding how to different bacterial strains are provided.
Microbiology Study Questions
21. Explain the purpose of Bergey's Manual and differentiate the types.
22. Describe how staining and biochemical tests are used to identify bacteria.
23. Differentiate between Western blotting and Southern blotting.
24. Explain how serological tests and phage typing can be used to identify an unknown organism.
25. Describe how a new bacterium can be classified by the following molecular methods: DNA base composition, rRNA sequencing, DNA fingerprinting, PCR, and nucleic acid hybridization.
26. Describe the defining characteristics of cyanobacteria, fungi, platyhelminths, cestodes, houseflies, algae, archaezoa, microspora, yeasts, helminths, protozoa
27. Differentiate between an intermediate host and a definitive host and give an example of what parasite stage is seen in humans as intermediate and definitive hosts.
28. Describe the defining characteristics of Bacteroides, Thiobacillus, Bacillales, Neisseria, Pseudomonas.
29. Differentiate between a tick and a mosquito, and name a disease transmitted by each.
30. Discuss the relationship of DNA- and RNA-containing viruses to cancer.