I give you a very expensive strain of E. coli containing 10^12 CFU/ml and tell you that I need exactly 10ml of the same E. coli culture at a concentration of 10^4 CFU/ml (I don't want any excess culture). Using a dilution series, how would you get the correct concentration and volume of bacteria? Make this a realistic scenario.© BrainMass Inc. brainmass.com October 24, 2018, 8:28 pm ad1c9bdddf
If we start with 10^14 CFU/ml, then we need to dilute it appropriately to end up with 10^4 CFU/ml.
If we take 1 ml of the original culture and dilute it into 1000 mL, then we ...
Dilution Series for Liquid Culture of Staphylococcus Aureus
If you had a liquid culture of Staphylococcus aureus and transferred 2ml to test tube "R", which contains 8ml of nutrient broth, what would be the final dilution (in simplest terms) of the culture in test tube "R"? From the culture in "R" make a 1:15 dilution in tube "S". What is the final dilution of the culture in tube "S"?View Full Posting Details