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# Designing of protocol to prepare dilution series

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A culture was determined to have 5.4 x 10^6 CFU/ml. Design a stepwise protocol to determine this number. For practice use different volumes of diluent and diluate.

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The solution discusses designing of protocol to prepare the dilution series.

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## Determine the glucose tolerance of two different patients by generating and using a standard curve to calculate the amount of glucose in their blood before and after eating.

This lab provided a 100mM Glucose standard solution which you are required to dilute with water to give final concentrations of 0,0.4,0.8,1.2,1.6 and 2.0 mmol/L glucose in a final volume 0.5ml. You will then use those concentrations to generate a standard curve using a spectrophotometer in order to calculate the amount of glucose in two different patient samples. Here are the absorbance-measurements of the six tubes: tube 1:0,tube 2:0.310, tube3:0.611, tube4:0.938, tube 5:1.016, tube6: 1.021

This solution will address the following:
1)produce a table of dilutions
2)prepare a 10-fold diluted 'patient sample ' from the above data to give 0.5ml final volume.Perform the 'patient samples' in duplicate.Absorbance for each solution taken at 550nm.
3)Results were obtained by measuring absorbances for the 2patients at time=0 and time=2hours to check glucose tolerance. So, time 0/patient 1 absorb=0.855 and 0.876 and patient 2 absorb=0.782 and 0.788 then at time 2hours we got for patient 1 absorb=0.748 and 0.752 and patient 2 absorb.=0.710 and 0.692.(remember is duplicated) From all this data use EXCEL to construct standard curves and determine the concentrations of the 2patient samples
4)Convert the values obtained in mmol/L into mg/dL and explain how
5)Use WHO guidelines for the diagnosis of diabetes to interprete the patients results during this Glucose Tolerance Test.
6)What component in Lucozade(given as a glucose resource) may have an adverse effect on the results of the Glucose Tolerance Test?

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