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Dilution Series

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1. If the original culture contains 5 x 10 ^9 cfu/ml, how many cfu/ml are in each tube? How many colonies are on each plate?

2. If tube C contains 60,000 cfu/ml, how many cfu/ml are in the original culture, how many cfu/ml are in the other tubes, and how many colonies are on each plate?

3. How would you inoculate a plate to obtain a 1:100 dilution?

4. How would you inoculate a plate to obtain a 1:50 dilution?

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Solution Summary

Detailed explanation of how a specific dilution series is made and answers to questions pertaining to it.

Solution Preview

Please see the attached file.

1. See attachment. For plates the quantity of cfu or colony forming units is the amount of colonies that will form.

2. If C= 5x10^9 cfu/ml and ...

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