Autoradiography depends upon particles emitted from radioactive atoms striking a photographic emulsion that lies on top of the tissue section. When the emulsion is developed, the site where the particle struck the emulsion is developed, the site where the particle struck the emulsion appears as a silver grain, as in figure 8.3a. how do you think the thickness of the section might affect the resolution of the technique, that is the ability to locate the precise site in the cell where radioactivity is incorporated?
To answer your question I will give you a little basic background on autoradiography, so that you will have a better understanding.
In short: Autoradiography is a visualizing technique that relies on the use of radioactive isotopes. The presence (energy emission) of radioactive isotopes can develop photographic film (much the same way light does). It can be used to visualize the location of nucleic acid, cell components, and in your case - proteins near the cytoplasmic membrane.
Autoradiography can be broken down into these general steps:
1. Living cells are briefly exposed to a specific radioactive compound. The compound is usually a radioactive element that is a component of what you are looking for.
2. The cells are then incubated for a variable period of time to allow then to incorporate the compound. Ex. Proteins will ...
The cytoplasmic membrane systems are examined.