That's all there is to it. If we plot this, the x-axis intercept will be the Bound(max).
We have to transform the data so that it makes sense. The raw data given in the question is "relative fluorescence" and "protein concentration." ...
Solution Summary
The solution provides the required sacatchard plot of the data in an attached excel sheet along with an explanation of what this represents and how to determine the maximum binding constant from the graph just formed.
... 15, 25 and 35 °C to determine the heat ... 6). The isoquinoline protease were determined under similar ... macromolecular structures by the maximum-likelihood method. ...
... Why do enzymes reach a maximum rate at high ... dimensional structure of many enzymes has been determined. ... S-shaped curve indicates cooperative binding of substrate ...
... the enzyme reaction; Vmax = the maximum velocity attainable ... the concentration has been determined according to ... In order to determine the extinction coefficient ...
... of quencher = 0. Use Equation 2 to determine JKL and ... value of F0 can be obtained by determining the intercept ... Next, using the value of Fo determined from the ...
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... The maximum particle size in the liquid must ... pathogens during ozonation will all determine the extended ... Temperature distribution must be determined in the food ...
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