If you were trying to determine the function of your favorite nucleoporins, your first experiment would be to see whether it binds to other nucleoporins. What type of experiment would you perform? What control would you use?
I know that you would do a Co-immunoprecipitation (CO-IP)because you can look for what else is bound to our protein of interest. CO-IP shows what things your protein would be in complex with while in the cell.
However, what control would I use?
Co-immunoprecipitation is one of the best ways to test for protein protein interactions. But you will need a positive control to insure that ...
Short paragraph with web references examining the appropriate control for a co-immunoprecipitation experiment.