Carbonic anhydrase of red blood cells, which has a molecular weight of 30,000, is among the most active known enzymes. It catalyzes the reversible hydration of CO2, which is important for the transport of carbon dioxide from the tissues to the lungs.
CO2 + H2O <--> H2CO3
If 10g of pure carbonic anhydrase catalyzes the hydration of 0.30g CO2 in one minute at 37 degrees C under optimal conditions, calculate the turnover number of carbonic anhydrase.
Solution is found by determining the number of moles of the enzyme and of CO2 and calculating the turnover number as how many moles of CO2 can be converted per mole of enzyme per minute.