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The purity and concentration of the stock solutions are configured.

Given the following spectrophotometric readings calculate the purity and concentration of the stock solutions.

Desired Ranges:
260/280=1.8-2.0
260/250=1.0-1.2
230/260=0.4-0.5

5uls of stock DNA were diluted with 995 HOH and measured.
Sample A280 A260 A250 A230 260/280 260/250 230/260 ug/ul of stock
1 0.189 0.350 0.313 0.151
2 0.054 0.095 0.084 0.041
3 0.089 0.159 0.142 0.072
4 0.111 0.201 0.180 0.092
5 0.130 0.237 0.212 0.108
6 0.128 0.231 0.206 0.102
7 0.032 0.048 0.043 0.029
8 0.057 0.101 0.090 0.046
9 0.141 0.256 0.230 0.117
10 0.122 0.220 0.196 0.098
11 0.086 0.151 0.135 0.072
12 0.149 0.270 0.242 0.134

Based on the above information, do you predict any difficulties in performing a restriction digest on any of the above samples? Explanation

Solution Summary

Calculate the purity and concentration of the stock solutions

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