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Calculate the Total Cell Number

You are given a 500ml culture of Euglena from which a 1 ml sample is taken and put in 49 ml of water. Absorbance was measured on this sample but the reading was off the scale. One ml of this solution is taken and diluted in another 249 ml of water, and this time the OD675nm gave the reading of .27. If the turbidity of .27 corresponds to 4X10^5 cell/ml. Calculate the total cell number in the original culture.

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We have 3 solutions : original, dilution 1 and dilution 2.

If the turbidity of 0.27 corresponds to 4X10^5 cell/ml, this ...

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