Separating Catenated Circular DNA Complexes
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Two circular DNA duplexes are catenated, or captured as interlocking rings. Draw a general mechanism that shows how these could be resolved into two free circles. You don't need to draw individual base pairs or a precise chemical mechanism of esterification by an active site tyrosine, but you do need to demonstrate generally how an enzyme could resolve these two tangled DNA strands (in this case, circles). Drawing the two duplexes in different colors or line widths will help a lot here. Must this be a type I or type II topoisomerase, and why? Must ATP have been used, and why? This answer must fit on one page, with the two explanations at the bottom of the page. (Be brief).
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The solution discusses separating catenated circuluar DNA complexes.
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7. Two circular DNA duplexes are catenated, or captured as interlocking rings. Draw a general mechanism that shows how these could be resolved into two free circles. You don't need to draw individual base pairs or a precise chemical mechanism of esterification by an active site tyrosine, but you do need to demonstrate generally how ...
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