Explore BrainMass
Share

3D Labs Analysis

This content was STOLEN from BrainMass.com - View the original, and get the already-completed solution here!

D Creation Lab (3DCL) specializes in innovative three dimensional color printing that enables consumers to make their computer designs tangible for various projects that may include prototypes often used for product development. This service is not exclusively extended to corporate executives; their services are also offered to every consumer who desires a 3D printing experience.

The management team operates inside the 3D Lab where Brian is the leader and the point of contact for all questions and concerns regarding consumer inquiries. The 3D Lab is located in Crown Lane Kidderminster, West Midlands.
Proprietary website: TerrainPrint.co.uk-specializes in customizing 3D landscapes anywhere in the world.

© BrainMass Inc. brainmass.com October 25, 2018, 5:25 am ad1c9bdddf
https://brainmass.com/business/leadership-skills/3d-labs-analysis-418944

Solution Preview

The Company

3D Creation Lab
http://3dcreationlab.co.uk/

3D Creation Lab (3DCL) specializes in innovative three dimensional color printing that enables consumers to make their computer designs tangible for various projects that may include prototypes often used for product development. This service is not exclusively extended to corporate executives; their services are also offered to every consumer who desires a 3D printing experience.

The management team operates inside the 3D Lab where Brian is the leader and the point of contact for all questions and concerns regarding consumer inquiries. The 3D Lab is located in Crown Lane Kidderminster, West Midlands.
Proprietary website: TerrainPrint.co.uk-specializes in customizing 3D landscapes anywhere in the world.

Organizational Structure:

Headquarters-is where all 3D printing projects are manufactured.
IT Department-coordinates all technological concerns regarding equipment maintenance, website maintenance, upgrade integration, and initiates innovative concepts for new products and services.

Administration-maintains organizational transactions that may include but are not limited to implementing new policies and procedures. Establish safety requirements for employees, and assurance of total quality management practices throughout the organization.

Management-monitors and supervises daily operations. Ensures that all orders are handled efficiently and in a timely manner. All inquiries will more than likely be directed to the management or team staff members depending on each circumstance.

Accounts Receivable and Accounts Payable (Billing) Department-This department will process payments from consumers and keep a records of all monetary transactions from consumers to 3DCL, and transactions from 3DCL to their creditors. This department will keep ...

Solution Summary

The solution discusses the 3D Creation Labs with a thorough analysis.

$2.19
See Also This Related BrainMass Solution

Please help with the following pre-lab questions regarding protein expression in my Molecular Biology Lab

PROTEIN EXPRESSION
Underlying molecular principles:
Explain the genetic components of the lac operon regulating the IPTG-inducible expression of T7 RNA polymerase in the TrcHisB/T7 and DH5 system

Hands-On Skills:
- Inoculate and grow a transformant DH5 cell line
- Assess the cell growth phase by absorbance at 600nm
- Induce protein expression by adding IPTG
- Harvest cells from a liquid culture
- Prepare one buffer solution to be used in a future laboratory class

I need help on the following two questions:
1. Perform a quick review of literature on recombinant protein expression in E. coli and indicate the protein yield reported in two research articles of your choice. Make sure you specify the units for the protein yield. For each article describe the nature of the protein that was overexpressed (membrane protein, cytosolic protein, ...). Estimate how much of your recombinant T7 RNA polymerase enzyme you might expect from 50ml of liquid culture by assuming a yield comparable to the ones reported in the two articles you selected. A hard copy of each of your two articles along with your answer should be handed in to your TA when entering the lab

2. 2. The overview figure provided in Experiment A shows the planning for the control aliquots to be assessed by SDS-PAGE in lab 6. Due to space limitation in the shaking incubator, some controls will be done collectively. Explain the information to be obtained from each of the two extra sets of controls to be done collectively:
a. The inoculated 50ml culture containing 200ug/ml ampicillin is to be divided in two fractions of 25ml. At time zero of the induction, the first 25ml fraction is to be induced with 1mM IPTG whereas only water is to be added to the second 25ml fraction. A 1ml aliquot is to be withdrawn from each of the two fractions at both time zero and after a 1.5 hr incubation. ( /2.5 MARKS)
b. A liquid culture of non transformed DH5 cells will be incubated without ampicillin, and a 1ml aliquot is to be collected at time zero and the very end of the induction treatment with IPTG.

Thanks again.

View Full Posting Details