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    Select a laboratory procedure or experiment and carry out a review of the hazards and risks associated with the procedure.
    1. using a template, write a report outlining,
    a. all the hazards and risks.
    b.what equipment, procedures etc. need to be in place in order to ensure it is carried out in a safe manner.

    Choose an area that has some hazards and risk.

    © BrainMass Inc. brainmass.com December 15, 2022, 5:59 pm ad1c9bdddf

    Solution Preview

    By template here, I am assuming that you are asked to pick any lab protocol in the field of biology and analyze it for hazards & risks, right?

    One protocol is used quite often in biochemistry is radiolabeling DNA oligos with P32-gamma-ATP for use in a gel shift assay. Since P32 is a radioactive isotope, one must take extra precautions when using it.

    The procedure for radiolabeling DNA:

    in an eppendorf tube, add known amount of your single-stranded DNA oligo
    add the polynucleotide kinase buffer
    add the appropriate amount of the P32-gamma ATP
    add the kinase
    incubate at 37C for 30min
    heat inactivate the kinase by heating the reaction at 65C for 20min
    allow the reaction to cool down
    apply the sample to a disposable spin column to remove the unincorporated gamma-ATP
    spin the sample through and collect it in an eppendorf tube
    dispose the tube in the proper waste bin
    to the tube of the sample, add the complementary DNA strand
    anneal the DNA by heating the sample ...

    Solution Summary

    A report using a template is provided. The equipment and procedure hazards are determined.