DNA Elongation

Related BrainMass Solutions

• An essay about transcription in eukaryotes.

  Elongation depends on Pol II escape by the polymerase getting past +30. Most of the data on the control of elongation has been derived from in vitro model systems where the rate of elongation is considerably slower than it is in vivo.

• Transcription of mRNA can be Terminated in Prokaryotic Cells

  Production of multiple copies of this recombinant DNA - Cloning d. Inserting the altered DNA into a recipient organism with the help of DNA ligase e. Screening of the transformed cell for the desired product (insulin).

• An essay on the transcription process

  Thymine rich sites found on eukaryotic DNA templates can actually block elongation by Pol II.

• Transcription and Translation

  Thymine rich sites found on eukaryotic DNA templates can actually block elongation by Pol II.

• Consensus sequences and the sigma factor

  Elongation occurs next, where the RNA polymerase moves along the DNA template strand and uses complementary bases to synthesize the RNA strand Once the RNA strand is fully synthesized, termination occurs, which prevents the RNA polymerase from acting

• Initiation of chromosomal DNA replication in a novel bacterium

  42816 Initiation of chromosomal DNA replication in a novel bacterium You are studying the initiation of chromosomal DNA replication in a novel bacterium that grows at 75°C.

• What is the Polymerase Chain Reaction?

  Elongation: In this step, the DNA polymerase will attach to the DNA strands that have primer bound to them and begin DNA replication of the sample DNA. This step is generally carried out at 72oC but may vary depending on the polymerase used.

• Transcription of DNA

  The process of adding nucleotides to the RNA strand is known as elongation, and in eukaryotes RNAP can build chains as long as 2.4 million nucleosides.

• PCR process

  Final elongation: This single step is occasionally performed at a temperature of 70-74°C for 5-15 minutes after the last PCR cycle to ensure that any remaining single-stranded DNA is fully extended.