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    Calculating the isoelectric point of a protein

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    I'm trying to calculate the isoelectric point of a protein. I understand that you need to substitute variations of pH until you find a pI that is close to zero. What I need is a detailed example of the process for a simple protein (say 4 amino acids, preferably charged). It would be helpful if you could also provide a similar protein and answer only so I can check my understanding. It would also be helpful if you could declare any constants (i.e. pH=5) in advance of both problems. Thanks very much.

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    Solution Summary

    This solution is provided in 488 words in an attached .doc file. It defines an isoelectric point and describes how charge in the acid is used to calculate pI.