Calculating the isoelectric point of a protein

Related BrainMass Solutions

• Definition of the isoelectric point of a protein

  1536 The definition of the Isoelectric point (pI) of a protein. What is the definition of the "isoelectric point" (pI) of a protein?

• Isoelectric points of proteins and exchange columns

  Also we explain how changes in the pH of the buffer containing a protein will alter its charge if we know its isoelectric point.

• PI (isoelectric poin)

  All proteins have a characteristic pI, or isoelectric point, at which the protein is neutrally charged overal (ie. the positive and negative charges on the protein balance each other).

• Galactose represser protein

  As you know, all proteins possess a net charge in solution, primarily dependent upon three things: the pH of the solution, their structure, and their isoelectric point (or pI).

• Various Biological and Organic Chemistry Questions

  Given that Serine is a polar neutral amino acid, it will prefer to be at the isoelectric point at pHs that are slightly larger or smaller than it isoelectric point pH.

• Polymer identification & protein adsorption

  If the overall charge of protein is zero, i.e. at isoelectric point, the electrostatic interaction between protein and polymer surface may still exist, because the charge distribution on the protein surface is not, as a rule, uniform.

• DNA Sequence Variation, Epigenetics, and Gel Electrophoresis

  It's highly unlikely that two proteins will have the exact same molecular weight AND isoelectric point.

• Determining the subunit composition of a protein from data

  354967 Determining the subunit composition of a protein from data Would you help me with the following problem? You isolate a new protein.

• Action of Trypsin on Peptides

  Each of those functional groups has a pKa point, at which it is not charged. Averaging the pKa points of all the functional groups of a fragment gives the isoelectric point. At this isoelectric point, it is neutral.