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E. coli; Amino Acid Alanine; Carcinogen; Mutagen ... etc

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Subject: There is a strain of E. coli that is unable to make its own amino acid alanine.

I am answering practice questions at the end of the chapter to get ready for a test.
This is a senario given that if I allow this bacterial strain to grow in medium containing alanine and then plate 10 to the 6th power cells on a petri dish containing medium with no alanine, I observe that an average of two colonies per plate will appear.
When you add blue dye #4 to the growth medium, twenty-two colonies appear. Can I conclude That my culture is contaminated with a phage? That blue dye #4 is a carcinogen? E. coli needs blue dye #4 for growth? Blue dye #4 is a mutagen? Blue dye #4 causes base substitutions? Alanine is a potential carcinogen?

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That my culture is contaminated with a phage? No.

That blue dye #4 is a carcinogen? I don't believe that bacteria get cancer! Seriously though, just because blue dye #4 is a mutagen (see below) doesn't mean that it causes cancer.

E. coli needs blue ...

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